In some experiments, HMCL-TR-shD1 had been also cotransduced with a lentivirus containing DEPDC1A gene under the control of Tet operator (HMCL-TR-DEP-shD1)
The lentivirus expressing shDEPDC1A or DEPDC1A have been produced employing Invitrogen methodology(Invitrogen, Carlsbad, Usa)....