Metal-organic frameworks (MOFs) have emerged as a promising class of microporous materials due to their high surface area, tunable pore size, and versatile functionality. These properties make them ideal candidates for solid-phase microextraction (SPME) coatings, particularly for the enrichment of trace volatile organic compounds (VOCs) in complex biological matrices such as exhaled breath. However, conventional MOF-based SPME coatings are predominantly derived from single-linker structures, limiting their ability to simultaneously enrich both polar and nonpolar analytes. This restriction arises because the selectivity of these coatings is primarily governed by the polarity of the linker molecules, which restricts their applicability in real-world samples with diverse chemical profiles. To overcome this limitation, we developed a hybrid Zeolitic Imidazolate Framework-8-90 (ZIF-8-90) coating on etched stainless steel fibers using a sol-gel method.PEG10 Antibody Autophagy ZIF-8-90 is constructed from a mixture of 2-methylimidazole (2-MeIM) and imidazolate-2-carboxyaldehyde (OHC-IM) linkers coordinated with Zn²⁺ ions, resulting in a framework that incorporates both methyl and aldehyde functional groups. This dual functionality enables synergistic interactions with a wide range of VOCs, including hydrophilic (ethanol, acetone, hexanol, hexanal) and hydrophobic (nonane, heptane, decane, isoprene) biomarkers.
The fabricated ZIF-8-90-coated fiber demonstrated excellent extraction performance across all eight target analytes. It exhibited high enrichment efficiency, broad linear dynamic range (three orders of magnitude), and low detection limits ranging from 0.82 to 2.64 μg L⁻¹. The relative standard deviation (RSD) for six consecutive extractions using the same fiber was between 2.5% and 7.3%, indicating outstanding repeatability. Inter-fiber reproducibility among three independently prepared fibers ranged from 4.8% to 12.0% (RSD), confirming good batch-to-batch consistency. Notably, the fiber maintained its performance over at least 120 extraction-desorption-conditioning cycles without significant loss in sensitivity or precision, highlighting its robustness and long service life.Neurogenin 3 Antibody In Vivo The enhanced performance is attributed to the combined effects of the methyl groups (favoring hydrophobic interactions) and aldehyde groups (capable of hydrogen bonding with polar species), along with the well-defined microporous structure of ZIF-8-90 (pore size ~4–5 Å), which allows efficient diffusion and adsorption of small molecule analytes.PMID:35078977
The method was successfully applied to real exhaled breath samples collected from six gastric cancer patients and six healthy volunteers. Quantitative analysis revealed significantly elevated levels of ethanol, acetone, hexanal, hexanol, nonane, and decane in cancer patients compared to controls, while isoprene levels were relatively lower. Recovery studies using spiked standards showed recoveries between 88% and 106%, confirming the accuracy and reliability of the method. In comparison to commercial PDMS and PA fibers, as well as single-linker ZIF-8 and ZIF-90 coatings, the ZIF-8-90-coated fiber consistently outperformed all others in terms of enrichment capacity for both polar and nonpolar targets. This work establishes a new paradigm for designing multifunctional MOF coatings through rational hybridization, offering a powerful tool for non-invasive, early-stage diagnosis of gastric cancer via breath analysis.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
