Name :
SARS-CoV-2 NSP10 Protein (His)
Description :
NSP10 is a major regulator of coronavirus replicase function. NSP10 contains two zinc fingers and binds and stimulates both NSP14 and NSP16 activities. Researchers has found that the nsp10 surface that interacts with nsp14 and nsp16 and possibly other subunits of the viral replication complex may be a target for the development of antiviral compounds against pathogenic coronaviruses.
Species :
SARS-CoV-2
Uniprotkb :
E. coli
Tag :
Tag Free
Synonyms :
Construction :
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) NSP10 (YP_009725306.1) (Ala1-Gln139) was expressed with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.
Protein Purity :
> 95 % as determined by SDS-PAGE.
Molecular Weight :
Approxiamtely 15.7 kDa
Endotoxin :
Please contact us for more information.
Formulatione :
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background :
NSP10 is a major regulator of coronavirus replicase function. NSP10 contains two zinc fingers and binds and stimulates both NSP14 and NSP16 activities. Researchers has found that the nsp10 surface that interacts with nsp14 and nsp16 and possibly other subunits of the viral replication complex may be a target for the development of antiviral compounds against pathogenic coronaviruses.
References and Literature :
1. Mickaël Bouvet,et al.Coronavirus Nsp10, a Critical Co-factor for Activation of Multiple Replicative Enzymes.THE JOURNAL OF BIOLOGICAL CHEMISTRY.2014Yuanyuan Ma,et al.Structural basis and functional analysis of the SARS coronavirus nsp14-nsp10 complex. Proc Natl Acad Sci USA. 2015Etienne Decroly,et al.Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2′-O-methyltransferase nsp10/nsp16 complex. PLoS Pathog.2011
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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