Name :
Human Siglec-3 / CD33 Protein, His Tag (MALS verified)
Background :
Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Iglike lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
Biological Activity :
Immobilized Anti-Human CD33 MAb, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human Siglec-3, His Tag (Cat. No. CD3-H5226) with a linear range of 0.1-2 ng/mL (QC tested).
Species :
Source :
Human Siglec-3, His Tag (CD3-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 18 – His 259 (Accession # AAH28152.1 ).
Tag :
Synonyms :
（Synonym）CD33,SIGLEC3,gp67
Purity :
（Purity）>95% as determined by SDS-PAGE.
Storage and Stability :
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin Level :
（Endotoxin）Less than 0.1 EU per μg by the LAL method.
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Protein Structure :
This protein carries a polyhistidine tag at the C-terminus.
Refactoring Approach :
Please see Certificate of Analysis for specific instructions.
Protein Labeling :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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