Ifficult to prepare and we currently only offer ddC. An alternative approach to the preparation of these oligos is to synthesize in the 5′-3′ direction, using 5’supports and phosphoramidites, with the 2′,3′-dideoxynucleoside 5’phosphoramidite being added to the 3’terminus in the last cycle. Unfortunately, since the 2′,3′-dideoxynucleoside 5’phosphoramidite has no DMT group, this approach is incompatible with DMT-on purification techniques. Also, since failure sequences contain a 3′-OH group, it is imperative that they be removed from the product by ionexchange HPLC or gel electrophoresis. The structures of the 2′,3’dideoxynucleoside monomers are shown, (4) – (7), Figure 3.
PRODUCT UPDATE – WHICH 5′-AMINO-MODIFIER
Our most popular 5′-aminomodifier is the C6 version, available with a monomethoxytrityl (MMT) or a trifluoroacetyl (TFA) protecting group. Which is appropriate for what set of circumstances If you wish to purify the 5′-aminomodified oligonucleotide, the MMT group is preferable since the oligo can be easily purified by reverse phase techniques. The MMT group is then removed with aqueous acid. Also, the MMT group can be removed by extended deblocking on the synthesizer, allowing a solid-phase conjugation of a tag containing an activated carboxylic acid. However, the conjugate must be stable to the subsequent conditions of cleavage and deprotection. The base-labile TFA group is preferred if the amino-modified oligo is not going to be purified prior to the conjugation reaction. It is logical to assume that only the amino-modified oligo is full-length and, therefore, that the conjugation reaction will select for only the full-length oligo.
ORDERING INFORMATION
Item 5′-Amino-Modifier C6 5′-Amino-Modifier C6-TFA Catalog No. 10-1906-90 10-1906-02 10-1916-90 10-1916-02 Pack 100 ole 0.25g 100 ole 0.25g Price($) 60.00 200.00 30.00 100.00
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NOVEL UNIVERSAL SUPPORT FEATURES RAPID AMIDEASSISTED DEPHOSPHORYLATION
The vast majority of oligonucleotide syntheses are carried out on supports to which the first nucleoside has been pre-attached.1825355-56-3 Biological Activity Following routine cleavage and deprotection, this nucleoside becomes the terminal nucleoside of the target oligonucleotide.150399-23-8 manufacturer For standard DNA and RNA synthesis, this requires an inventory of four deoxynucleoside and four ribonucleoside supports.PMID:29939554 A universal support (a support without the first nucleoside attached) offers the following advantages: eliminates the need for an inventory of nucleoside supports. minimizes the possibility of error in the selection of the correct support type. reduces time and eliminates the possible error in the generation of an array of nucleoside supports in 96 well synthesizers. allows the preparation of oligonucleotides containing a 3′-terminal nucleoside which is not available as a support. Since these are major benefits, why is the use of a universal support not, well, universal Indeed, universal-type supports are standard in the synthesis of amino-, thiol-, and other modified oligonucleotides. The major hurdle to overcome is to find conditions to eliminate the terminal phosphate, produced from the first nucleoside phosphoramidite addition cycle, to the required terminal hydroxyl group. Let’s look at the problem in detail. Universal supports are based on the ribonucleoside elimination model exemplified by our Universal Support (.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
