482nm is 78,300 L/mol.cm. Our experimental work has shown that these products are fully compatible with regular oligonucleotide synthesis and deprotection. A coupling time of 12 minutes is recommended for the phosphoramidite, while the support does not require any changes of cycle. Oligonucleotides containing the GalNAc group can be deprotected using standard procedures during which the acetyl protecting groups on the GalNAc group are removed. The following conditions have been tested and shown to be acceptable:
The chromatogram in Figure 6 demonstrates the high purity of 5′-GalNAc C3-T 6 synthesized using a 12 minute coupling time. For the ESI spectrum (Figure 6, Inset), the target mass for the GalNAc C3-T6 is 2372.80 Da (Obs: 2372.7). Note: the higher molecular weight species with a mass of 2538.2 Da is the HFIPA adduct. As shown in Figure 7, we have demonstrated that 5′-GalNAc C3 phosphoramidite can be used to prepare oligonucleotides with three consecutive GalNAc additions at the 5′ terminus. Glen Research is delighted to be able to offer these GalNAc C3 products under an agreement with AM Chemicals LLC. We thank Andrei Guzaev for helpful discussions while preparing this article to introduce these products for sale.
SEQUENCE MODIFICATION USING GLEN RESEARCH’S 5-MODIFIED dU FAMILY
Glen Research’s first nucleoside for sequence modification, Amino-Modifier C6 dT (10-1039), was introduced in October 1989 and has led to a family of analogues that are useful for a variety of purposes. Although these are truly dU analogues, they behave as dT in primers and probes.838818-26-1 Formula By using the 5 position of the dU base (1), the attached tags project into the major groove of double stranded DNA where they are readily available for detection. Even extremely large molecules, like the enzyme horseradish peroxidase, can be attached at that position with minimal perturbation of normal hybridization. In this article, we will cover familial subsets designed for modification, fluorescent labelling, quenchers and click chemistry.
Amino-Modifier C6 dT (10-1039) has a long spacer designed to project the tag beyond the hybridization limit, whereas NH closely related Amino-Modifier C2 dT O (10-1037) allows the tag to interact with adjacent DNA strands. In both cases, the primary amino group is revealed on deprotection, ready NH post synthesis for conjugation to a suitable tag, e.g., an O N-hydroxysuccinimide (NHS) ester. In both cases, the amino group is protected as a trifluoroacetate (TFA). We have never found conditions which N N + N allow the TFA group to be removed from an N amino-modifier while the oligonucleotide remains attached to the support. We are able to solve this problem by using a 9-fluorenylmethoxycarbonyl (Fmoc) protecting group. The Fmoc group can be removed on the synthesis column to allow on-column reaction with a variety of activated esters.196618-13-0 Biological Activity Fmoc Amino-Modifier C6 dT (10-1536) is our dT analogue for this purpose.PMID:30335334 Carboxy-dT (10-1035) is hydrolyzed during deprotection with 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature and can be
coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate NHS ester. With the introduction of NHS-CarboxydT (10-1535), it is possible to label one or multiple sites within an oligonucleotide with an amino tag while still on the support. This opens up the possibility to label any number of different dyes or molecules within an.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
