Inc. to recommence the supply of these two valuable products. We believe these will be a welcome addition to the selection of modified bases that affect hybridization. References:
C-5 propynyl Pyrimidine Analogues Substitution of C-5 propynyl-dC (pdC) for dC and C-5 propynyl-dU (pdU) for dT1 are effective strategies to enhance base pairing. This increase in hybridization efficiency is due to the hydrophobic nature of the groups at the C-5 position which helps to exclude water molecules from the duplex. Using these base substitutions, duplex stability and therefore melting temperatures
Pyrrolo-CTP Pyrrolo-dC is a fluorescent nucleoside that codes as dC and base pairs efficiently with dG. We have published a preliminary report1 on the chemistry of pyrrolo-dC and further details of the chemistry and biology are currently in press. Preliminary evidence indicates that pyrrolo-dC triphosphate is an excellent substrate for Taq, Pfu and Vent polymerases and is incorporated specifically opposite dG. Pyrrolo-dCTP has been available for some time and is in use in biological assays. We are now introducing pyrroloCTP, the ribonucleoside triphosphate. We anticipate that the addition of a fluorescent ribonucleotide with fluorescence exquisitely sensitive to its environment would be of great interest for RNA structural research. The pyrrolo-C project is a joint development by Berry and Associates (http:// berryassoc) and Glen Research.
In all 20 years of Glen Research’s existence, we have heard prediction after prediction that RNA synthesis was about to enjoy explosive growth. Although activity certainly increased slightly over the years thanks to studies on RNA structure and function relative to RNA viral activity, t-RNA and ribozymes (among others) growth could never have been defined as explosive. Finally, these predictions seem to be coming to fruition thanks to the advent of siRNA. Currently, researchers seem to be happy with the ready availability of unmodified siRNA but we predict that interest will continue to rise in the ability to use base, sugar and phosphate modifications to optimize siRNA activity. In this short article, we will focus on base modifications that are potentially useful in siRNA research. In the following article on Page 3, we will highlight a modified RNA base with significant biological interest and potential: 1-Methyl-Adenosine (m1A), the phosphoramidite of which is shown in Figure 1 on this page. A quick glance through our catalog or a browse through our web site quickly reveals a significant selection of base modified and minor RNA monomers available from stock. Here is the current list:
7-Deaza-Adenosine (10-3001) 8-Aza-7-deaza-Adenosine (10-3083) Zebularine (10-3011) Pyridin-2-one Ribonucleoside (10-3012) Nebularine (Purine Ribonucleoside) (10-3041) PseudoUridine (10-3055)
Pyrrolo-Cytidine (10-3017) Inosine (10-3040) 5-Methyl-Uridine (ribo version of Thymidine) (10-3050) 4-Thio-Uridine (10-3052) 5-Methyl-Cytidine (10-3064) 2-Aminopurine Ribonucleoside (10-3070) 6-Thio-Guanosine (10-3072) 2,6-Diaminopurine Ribonucleoside (2-AminoAdenosine) (10-3084) 5-Bromo-Uridine (10-3090) 5-Iodo-Uridine (10-3091) Amino-Modifier C6-U (10-3039) rSpacer (10-3914)
The structures of these RNA phosphoramidites are shown in Figure 2, Page 2 and the following is a very brief introduction to each of these monomers.67-68-5 site Adenosine Analogues 7-Deaza-Adenosine is lacking nitrogen at the 7-position, which is replaced by carbon.557795-19-4 References The N7 position in adenosine.PMID:30480963 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
