Ster mix (Applied Biosystems, Foster City, CA; 4309155) having a real-time PCR instrument (Applied Biosystems, 7200). The sequences of primers were as follows (five to 3)40: CaV1.1-forward: GTTACATGAGCTGGATCACACAG; CaV1.1-reverse: ATGAGCATTTCGA-TGGTGAAG;…