Pared as previously described (Sailer et al, 2002) and solubilized with ComplexioLyte 47 (CL-47, Logopharm GmbH) for 30 mins on ice (at a concentration of 1.25 mg/ml). Following clearing by…
Pared as previously described (Sailer et al, 2002) and solubilized with ComplexioLyte 47 (CL-47, Logopharm GmbH) for 30 mins on ice (at a concentration of 1.25 mg/ml). Following clearing by…
Ntitative real-time PCR of Itgae expression in purified TCR+CD4+ lymphocytes from spleen (SPL), lamina propria (LPL) or intra-epithelium (IEL). Data are representative final results of at least three independent experiments.…