The sections were then washed thrice with PBS, incubated with FITC labeled respective secondary antibody (Santa-cruz biotech) for 1 hour in a dim was saved at 280uC until employed
The blots were then incubated with secondary IgG HRP conjugated goat anti-rabbit (one: 5000) or donkey antimouse (1: 5000) antibodies (Santa Cruz Biotechnology, CA, United states) in PBS at area…