The cells had been plated on collagen-coated coverslips (50 mg/cm2) and fastened twenty min after plating
HEK293 cells had been transfected with pCMV5-FLAG-ARHGAP22. After 24 h, the cells were washed two times with ten ml of Phosphate-buffered saline (PBS) and incubated with 1 mM DSP for…