These final results suggest that phosphorylated PPPSPXS peptide inhibited b-catenin phosphorylation by GSK3 through a manner that is impartial of PPPSPXS-binding to Axin, most likely by inhibiting GSK3 straight
In vitro reconstitution of Axin-dependent and CK1 priming-dependent b-catenin phosphorylation by GSK3. A. Recombinant GST-b-catenin, Flag-CK1, MBP-Axin, and His-GSK3 proteins were being expressed in bacteria or insect cells and purified…