The proposed topology of OCTN2 assumes each N- and C-termini to be localized in the cytoplasm. We cloned cDNA coding rOctn2 with C-terminally fused 3xFLAG peptide and stably expressed it in HEK293 cells
Evaluation of Octn2 phosphorylation and plasma membrane existence. The cells were incubated either devoid of any additions, or with PMA or Bis II and PMA, as described in Resources and…