Horseradish peroxidase-conjugated secondary antibodies were detected making use of ECL reagents (Pierce)
HT-29Cl16E cells (Ephyscience) ended up cultured in Dulbecco’s modified Eagle’s medium (four.five g/liter glucose) supplemented with glutamax, ten% calf serum, 100 mg/ml streptomycin, and a hundred U/ ml penicillin at…