The corresponding PrPC represented mono- or non-glycosylated PrPC in MDCK cells (Fig. 1). PrPC glycomutants and wild-sort PrPC (PrPCWT) contained the 3F4 epitope tag making it possible for discrimination of overexpressed from endogenous PrPC [32]
One of the needs of our study was to establish the purpose of the glycans in sorting of mouse PrPC. We consequently generated stably expressing PrPC mutants in which the…