This is probably dues to diverse post-translational modifications (see EF1 in Determine 1A, B) or the likely presence of multiple isoforms in the egg extract in the case of enolase
First we injected glucose-6-phosphate (G6P), elevating the oocyte G6P concentration by one.38 mM, as this is the final solution of gluconeogenesis. The intracellular focus of the G6P in the oocyte…